cd105 polyclonal antibodies  (Bioss)

Journal: Veterinary World

Article Title: Mitigation of mycotoxin residues and activation of endogenous stem cells in broiler chickens using a toxin binder: Implications for meat safety and performance enhancement

doi: 10.14202/vetworld.2025.1850-1862

Figure Lengend Snippet: Mean of CD34+, CD45+, CD105− with flow cytometry.

Article Snippet: Extracellular staining: Cell pellets were incubated with 50 μL of CD34, CD45, and CD105 polyclonal antibodies (Bioss Inc., USA) for 20 min in the dark at 20°C Intracellular staining: Fixation and permeabilization were performed using BioLegend reagents before secondary antibody incubation Instrumentation: Flow cytometric analysis was conducted on a BD FACSCalibur system (BD Biosciences, USA) with daily calibration and appropriate gating for CD34+, CD45+, and CD105− populations.

Techniques: Flow Cytometry

Journal: Bioengineering

Article Title: Evaluation of Skin Wound Healing with Biosheets Containing Somatic Stem Cells in a Dog Model: A Pilot Study

doi: 10.3390/bioengineering11050435

Figure Lengend Snippet: Macroscopic ( a ), H&E-stained ( b ), and Masson’s trichrome-stained ( c ) sections of biosheets formed in molds with different pore shapes. Collagen-rich tissue ( 1 ) and fibrin clot ( 2 ) positions formed in the molds with Y- and O/Y-shaped pores and their high-magnification images ( 1-1 , 1-2 ) and SSEA-4 and CD105 antigen-immunostained images ( 2-1 , 2-2 ).

Article Snippet: Subsequently, the sections were washed in distilled water twice for 10 min and blocked in 1% bovine serum albumin (Wako pure chemicals) in PBS at 24–26 °C for 1 h and incubated with anti-SSEA4 mouse monoclonal antibody (1:100, ab16287; Abcam, Cambridge, UK) and anti-CD105 rabbit polyclonal antibody (1:200, bs-0579R; Bioss Inc., Boston, MA, USA) overnight at 4 °C.

Techniques: Staining

Journal: Materials Today Bio

Article Title: Vascular tissue reconstruction by monocyte subpopulations on small-diameter acellular grafts via integrin activation

doi: 10.1016/j.mtbio.2023.100847

Figure Lengend Snippet: The surface maker for the antibody used in this study.

Article Snippet: The cells were stained with PE-conjugated anti-CD31 antibody (MCA1746PET, Bio-Rad Laboratories, Montreal, Quebec), anti-CD34 antibody (bs-0646R-PE, Bioss Antibody Inc.), anti-CD105 antibody (bs-0579R-PE, Bioss Antibody Inc.), and anti-Flk-1 antibody (bs-0565R-PE, Bioss Antibody Inc), anti-CD163 antibody (bs-2527R-PE, Bioss Antibody Inc.), anti-CD14 antibody (MCA1218F, Bio-Rad Laboratories, Inc., Hercules, CA), and anti-CD16 antibody (MCA1971PE, Bio-Rad Laboratories, Inc.).

Techniques: Expressing, Marker

Journal: Materials Today Bio

Article Title: Vascular tissue reconstruction by monocyte subpopulations on small-diameter acellular grafts via integrin activation

doi: 10.1016/j.mtbio.2023.100847

Figure Lengend Snippet: Blood response to the luminal surface of the P-graft and Un-graft. a, Gross images and SEM observation of the luminal surface of the P-graft and Un-graft. Blood was circulated for 1 h by a cardiopulmonary pump system. The red arrowhead indicates clot deposition. b , SEM images of the luminal surface of the P-graft after transplantation for 3 h, 1 day, and 3 days. c , Cross-section images of the P-graft after transplantation for 1 day. The sections were stained with HE, anti-CD31, anti-CD34, anti-CD105, and anti-Flk-1 antibodies. Asterisks indicate the luminal side. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The cells were stained with PE-conjugated anti-CD31 antibody (MCA1746PET, Bio-Rad Laboratories, Montreal, Quebec), anti-CD34 antibody (bs-0646R-PE, Bioss Antibody Inc.), anti-CD105 antibody (bs-0579R-PE, Bioss Antibody Inc.), and anti-Flk-1 antibody (bs-0565R-PE, Bioss Antibody Inc), anti-CD163 antibody (bs-2527R-PE, Bioss Antibody Inc.), anti-CD14 antibody (MCA1218F, Bio-Rad Laboratories, Inc., Hercules, CA), and anti-CD16 antibody (MCA1971PE, Bio-Rad Laboratories, Inc.).

Techniques: Transplantation Assay, Staining

Journal: Materials Today Bio

Article Title: Vascular tissue reconstruction by monocyte subpopulations on small-diameter acellular grafts via integrin activation

doi: 10.1016/j.mtbio.2023.100847

Figure Lengend Snippet: Surface marker analysis of captured cells and porcine monocyte. The captured cells were isolated from the 3 h transplantation P-graft. a , Expression levels of CD31, CD34, CD105 and Flk-1 in CC-3H were indicated. b , Two-dimensional expression patterns of CD163/CD14 and CD16/CD14 in the captured cells were plotted. c , Two-dimensional expression patterns of CD163/CD14 and CD16/CD14 in the porcine monocyte were plotted. The MoN and MoP populations were expressed CD14 Low /CD16 Low /CD163 + and CD14 + /CD16 + /CD163 + , respectively.

Article Snippet: The cells were stained with PE-conjugated anti-CD31 antibody (MCA1746PET, Bio-Rad Laboratories, Montreal, Quebec), anti-CD34 antibody (bs-0646R-PE, Bioss Antibody Inc.), anti-CD105 antibody (bs-0579R-PE, Bioss Antibody Inc.), and anti-Flk-1 antibody (bs-0565R-PE, Bioss Antibody Inc), anti-CD163 antibody (bs-2527R-PE, Bioss Antibody Inc.), anti-CD14 antibody (MCA1218F, Bio-Rad Laboratories, Inc., Hercules, CA), and anti-CD16 antibody (MCA1971PE, Bio-Rad Laboratories, Inc.).

Techniques: Marker, Isolation, Transplantation Assay, Expressing

Journal: Materials Today Bio

Article Title: Vascular tissue reconstruction by monocyte subpopulations on small-diameter acellular grafts via integrin activation

doi: 10.1016/j.mtbio.2023.100847

Figure Lengend Snippet: Outgrowth capacity and surface marker analysis of captured cells a , Outgrowth capacity was evaluated on the cell culture plate. The cells were grown from the tissue after 3 days. b,c, Two-type cells were isolated from the single colony cultivation. The cells were classified into ( b) filopodia-shaped (CC-3D 1 ) and (c) spindle-shaped (CC-3D 2 ) cells based on the difference in morphology. These cells were expressed the surface maker of CD16/CD14. d , e , Surface marker expression of CD31, CD34, CD105, and Flk-1 on ( d ) CC-3D 1 and ( e ) CC-3D 2 were indicated. f , Di-ac-LDL uptake of the mixture of CC-3D 1 and CC-3D 2 was compared to the fibroblast and endothelial cells on CLSM observation.

Article Snippet: The cells were stained with PE-conjugated anti-CD31 antibody (MCA1746PET, Bio-Rad Laboratories, Montreal, Quebec), anti-CD34 antibody (bs-0646R-PE, Bioss Antibody Inc.), anti-CD105 antibody (bs-0579R-PE, Bioss Antibody Inc.), and anti-Flk-1 antibody (bs-0565R-PE, Bioss Antibody Inc), anti-CD163 antibody (bs-2527R-PE, Bioss Antibody Inc.), anti-CD14 antibody (MCA1218F, Bio-Rad Laboratories, Inc., Hercules, CA), and anti-CD16 antibody (MCA1971PE, Bio-Rad Laboratories, Inc.).

Techniques: Marker, Cell Culture, Isolation, Expressing

Journal: Materials Today Bio

Article Title: Vascular tissue reconstruction by monocyte subpopulations on small-diameter acellular grafts via integrin activation

doi: 10.1016/j.mtbio.2023.100847

Figure Lengend Snippet: Blood response to the luminal surface of the P-graft and Un-graft. a, Gross images and SEM observation of the luminal surface of the P-graft and Un-graft. Blood was circulated for 1 h by a cardiopulmonary pump system. The red arrowhead indicates clot deposition. b , SEM images of the luminal surface of the P-graft after transplantation for 3 h, 1 day, and 3 days. c , Cross-section images of the P-graft after transplantation for 1 day. The sections were stained with HE, anti-CD31, anti-CD34, anti-CD105, and anti-Flk-1 antibodies. Asterisks indicate the luminal side. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Immunostaining was also carried out using anti-CD31 (MCA1746, Bio-Rad Laboratories, Montreal, Quebec), CD34 (bs-0646R, Bioss Antibody Inc., Boston, MA), CD105 (bs-0579R, Bioss Antibody Inc.), and Flk-1 antibodies (bs-0565R, Bioss antibody Inc.). summarizes the types and roles of the antibodies used in this study.

Techniques: Transplantation Assay, Staining

Journal: Materials Today Bio

Article Title: Vascular tissue reconstruction by monocyte subpopulations on small-diameter acellular grafts via integrin activation

doi: 10.1016/j.mtbio.2023.100847

Figure Lengend Snippet: Surface marker analysis of captured cells and porcine monocyte. The captured cells were isolated from the 3 h transplantation P-graft. a , Expression levels of CD31, CD34, CD105 and Flk-1 in CC-3H were indicated. b , Two-dimensional expression patterns of CD163/CD14 and CD16/CD14 in the captured cells were plotted. c , Two-dimensional expression patterns of CD163/CD14 and CD16/CD14 in the porcine monocyte were plotted. The MoN and MoP populations were expressed CD14 Low /CD16 Low /CD163 + and CD14 + /CD16 + /CD163 + , respectively.

Article Snippet: Immunostaining was also carried out using anti-CD31 (MCA1746, Bio-Rad Laboratories, Montreal, Quebec), CD34 (bs-0646R, Bioss Antibody Inc., Boston, MA), CD105 (bs-0579R, Bioss Antibody Inc.), and Flk-1 antibodies (bs-0565R, Bioss antibody Inc.). summarizes the types and roles of the antibodies used in this study.

Techniques: Marker, Isolation, Transplantation Assay, Expressing

Journal: Materials Today Bio

Article Title: Vascular tissue reconstruction by monocyte subpopulations on small-diameter acellular grafts via integrin activation

doi: 10.1016/j.mtbio.2023.100847

Figure Lengend Snippet: Outgrowth capacity and surface marker analysis of captured cells a , Outgrowth capacity was evaluated on the cell culture plate. The cells were grown from the tissue after 3 days. b,c, Two-type cells were isolated from the single colony cultivation. The cells were classified into ( b) filopodia-shaped (CC-3D 1 ) and (c) spindle-shaped (CC-3D 2 ) cells based on the difference in morphology. These cells were expressed the surface maker of CD16/CD14. d , e , Surface marker expression of CD31, CD34, CD105, and Flk-1 on ( d ) CC-3D 1 and ( e ) CC-3D 2 were indicated. f , Di-ac-LDL uptake of the mixture of CC-3D 1 and CC-3D 2 was compared to the fibroblast and endothelial cells on CLSM observation.

Article Snippet: Immunostaining was also carried out using anti-CD31 (MCA1746, Bio-Rad Laboratories, Montreal, Quebec), CD34 (bs-0646R, Bioss Antibody Inc., Boston, MA), CD105 (bs-0579R, Bioss Antibody Inc.), and Flk-1 antibodies (bs-0565R, Bioss antibody Inc.). summarizes the types and roles of the antibodies used in this study.

Techniques: Marker, Cell Culture, Isolation, Expressing